Journal: Brazilian Journal of Medical and Biological Research

Article Title: Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

doi: 10.1590/S0100-879X2010007500004

Figure Lengend Snippet: Figure 1. Expression of Chk1 and Chk2 mRNA induced by diallyl disulfide (DADS). BGC823 cells (1 x 106/mL) were treated with 15 mg/L DADS and expression of Chk1 and Chk2 mRNA was analyzed by RT-PCR. The relative amount of each mRNA was compared to β-actin. The data are reported as means ± SD (N = 3) on the right side of the figure. Each result is the aver age of two determinations. Left side of the figure: Lane 1, marker; lane 2, BGC823 cells; lanes 3 and 4, BGC823 cells treated with 15 mg/L DADS for 1 or 2 days. Right side of the figure: column 1 = BGC823 cells; column 2 = BGC823 cells treated with 15 mg/L DADS for 1 day; column 3 = BGC823 cells treated with 15 mg/L DADS for 2 days. There were no significant differences (t- test).

Article Snippet: The cyclin B1 and β-actin antibodies were from Boster Company (China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

doi: 10.1590/S0100-879X2010007500004

Figure Lengend Snippet: Figure 5. Expression of cyclin B1 by diallyl disulfide (DADS). BGC823 cells (1 x 106/mL) were treated with 15 mg/L DADS and expression of cyclin B1 was analyzed by Western blot. The rela tive amount of each protein was compared to β-actin. The data are reported as means ± SD (N = 3). Each result is the average of two determinations. *P < 0.05 vs control (t-test).

Article Snippet: The cyclin B1 and β-actin antibodies were from Boster Company (China).

Techniques: Expressing, Western Blot, Control

Journal: Oncologie

Article Title: Forkhead Box P4 promotes the proliferation of cells in colorectal adenocarcinoma

doi: 10.1515/oncologie-2023-0009

Figure Lengend Snippet: Figure 2: Correlation of FOXP4 and PCNA in CRAC.

Article Snippet: Rabbit anti-humanFOXP4 (16772-1-AP)wasprocured from Wuhan proteintech (Wuhan, China), and rabbit anti-human proliferating cell nuclear antigen (PCNA) (A00125) was procured from Wuhan Boster Biological Technology (Wuhan, China).

Techniques:

Journal: Science advances

Article Title: A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson's disease.

doi: 10.1126/sciadv.ado4927

Figure Lengend Snippet: Fig. 1. ATB NP–mediated photothermal stimulation promotes the activation and depolarization of TRPV1+ cells in vitro. (A and B) An illustration of the design of ATB NPs. ATB NPs were synthesized by conjugating the TRPV1 antibody against the TRPV1 extracellular domain [400 to 500 amino acids (aa)] and β-syn peptides to AuNSs. (C) Representative FTIR spectra of AuNSs, AT NPs, and ATB NPs. (D) Distribution of ATB NPs (1 × 109/ml) in primary dopaminergic neurons and HEK-293T cells transfected with or without TRPV1 plasmid, as shown by TEM. (E) Working model of ATB NP–mediated photothermal stimulation activating TRPV1 and Ca2+ influx in cells, as deter- mined by Fluo-4 AM probe. (F) Representative Ca2+ influx of control, PFF neurons, and PFF neurons treated with ATB NPs (1 × 109/ml) for 24 hours, before and after NIR laser irradiation (2 W, 20 ms per pulse, 20 Hz) for 1 min. Scale bars, 80 μm. (G) Ca2+ influx of HEK-293T cells transfected with TRPV1 plasmid or empty vector (set as control) incubated with ATB NPs, before and after NIR laser irradiation. Scale bars, 80 μm. (H) Whole-cell patch-clamp traces of TRPV1+ or TRPV1− cells in the presence or absence of ATB NPs for 24 hours and NIR laser treatment for 1 min (n = 5, five replicates). (I) Action potentials in PFF-treated DA neurons in response to ATB NPs and NIR laser irra- diation (n = 6, six replicates). (J) Representative immunofluorescence images of c-fos in primary dopaminergic neurons treated with PFF (2 μg/ml) for 3 days, followed by ATB NPs incubation for 24 hours and NIR laser irradiation for 1 min. Scale bars, 80 μm. The data are expressed as the means ± SD. Statistical significance was tested using a two-tailed t test and one-way analysis of variance (ANOVA) analysis. ***P < 0.001 and ****P < 0.0001. (A), (B), and (E) created using BioRender.com.

Article Snippet: 11, eado4927 (2025) 15 January 2025 12 of 16 with PBS and fixed for 15 min. After incubation with primary antibody of TRPV1 (A00128- 4, Boster Biological Technology) and Alexa Fluor 488–conjugated secondary antibody (A0423, Beyotime), the cells were washed with PBS and observed by laser reflection technology of confocal laser scanning microscope (STELLARIS 8, Leica).

Techniques: Activation Assay, In Vitro, Synthesized, Transfection, Plasmid Preparation, Control, Irradiation, Incubation, Patch Clamp, Immunofluorescence, Two Tailed Test

Journal: Science advances

Article Title: A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson's disease.

doi: 10.1126/sciadv.ado4927

Figure Lengend Snippet: Fig. 3. ATB NPs in the SN induce the activation of DA neurons and DA release in mice. (A) Representative immunofluorescence images showing TRPV1 expression in the TH-positive cells of SN in control and ATB-treated mice. ATB NP injection did not affect the TRPV1 expression in the mice. (B) Schematic representation of the treatment of C57BL/6J mice after stereotactic injection of PFF (5 μg) in the striatum for 3 months and subsequent ATB NPs (1 × 1011/ml, 5 μl) injection into the SN at different injection coordinates. (C) Distribution of ATB NPs in the brain, as determined by LA-ICP-MS after injection. Au appears in the SN zone. (D) Quantitative measurement of Au in the olfactory bulb, cortex, striatum, hippocampus, and SN in mice. The ATB NPs were stereotactically injected into the SN, and the content of Au in different regions of the mouse brain was measured at the indicated time points [0 to 5 weeks (w)] by ICP-MS (n = 4, biologically independent mice). (E) Schematic illustration of ATB NP injection into the SN 1 day before NIR irradiation and DA release measurement. SNc, substantia nigra pars compacta. (F) Temperature changes in the mouse brain, as recorded by thermal camera, after NIR irradiation for 3 min. (G) Representative confocal images showing the expression of c-fos in the SN of control, PFF mice, and PFF mice treated with ATB NPs and NIR laser. (H) Transient DA release in the striatum in response to ATB NPs and NIR stimulation in control and PFF-treated mice. A carbon fiber electrode was implanted into the striatum to monitor the DA levels in the striatum in real time using FSCV. All experiments were repeated at least three times. The data are expressed as the means ± SD. (B) and (E) created using BioRender.com.

Article Snippet: 11, eado4927 (2025) 15 January 2025 12 of 16 with PBS and fixed for 15 min. After incubation with primary antibody of TRPV1 (A00128- 4, Boster Biological Technology) and Alexa Fluor 488–conjugated secondary antibody (A0423, Beyotime), the cells were washed with PBS and observed by laser reflection technology of confocal laser scanning microscope (STELLARIS 8, Leica).

Techniques: Activation Assay, Immunofluorescence, Expressing, Control, Injection, Irradiation

Journal: Science advances

Article Title: A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson's disease.

doi: 10.1126/sciadv.ado4927

Figure Lengend Snippet: Fig. 6. Working model showing wireless DBS mediated by ATB NPs to reverse PD. (I) Pulsed NIR irradiation triggers the thermal activation of TRPV1 channels and Ca2+ influx into neurons to promote membrane depolarization. (II and III) NIR-induced β-syn peptide release into neurons causes a disaggregation of α-syn fibrils, combined with the thermal activation of CMA to clear the pathological α-syn fibrils. This therapy effectively reverses the symptoms of PD. Created using BioRender.com.

Article Snippet: 11, eado4927 (2025) 15 January 2025 12 of 16 with PBS and fixed for 15 min. After incubation with primary antibody of TRPV1 (A00128- 4, Boster Biological Technology) and Alexa Fluor 488–conjugated secondary antibody (A0423, Beyotime), the cells were washed with PBS and observed by laser reflection technology of confocal laser scanning microscope (STELLARIS 8, Leica).

Techniques: Irradiation, Activation Assay, Membrane